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These findings were similar whether data were assessed on an intention-to-treat (using imputation) or a per-protocol basis (data not shown).
The SNiP population includes almost all pregnant women living in "Ostvorpommern" who gave birth between May 1st, 2002 and November 30th, 2008 since data were assessed on all maternity wards in this rural region and the percentage of giving birth in a stationary setting is above 98%.
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Data was assessed on the basis of medical records and by questionnaires when necessary.
Quality of the data was assessed on diagnostic plots generated from the raw, non-processed data, as described [ 37].
The quality of microarray data was assessed on many levels, resulting in the omission of 5 of the 72 hybridizations from the analysis.
The quality of the microarray data was assessed on the basis of a report from the arrayQualityMetrics package [ 26] and by observation of the distribution of the log2 intensities on each array before and after normalization (Additional file 1A).
Quality of samples and data was assessed on the basis of parameters extracted from GCOS report (RPT) files and detection of array outliers performed with the dChip software [ 48] Version 2007.
In addition, students' seismological skills and their ability to work with multiple representations of data were assessed through hands-on skills assessments conducted in individual sessions with an experimenter.
Survival and economic data were assessed based on an 'intent to treat' analysis, thus analyzing patients according to the assigned treatment.
The concentration of Se in serum, biochemical parameters (total bilirubin, AST, ALT), AS (toxic metabolites of nitrogen oxide (NOx), superoxide dismutase (SOD) and malondialdehyde (MDA)) and clinical data were assessed before surgery and on the fifth day after surgery.
Nonparametric data were assessed by Kruskal-Wallis ANOVA on ranks followed by Dunn's post hoc analysis.
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