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Sequence data were assembled using NewBler assembler and Consed.
The GS FLX sequence data were assembled using Newbler assembly software.
The filtered and trimmed data were assembled using the combined assembly approach [ 23]: Two assemblies were generated, one using Newbler v2.6 [ 22] (with parameters -cdna -urt), the other using Mira v3.2.1 [ 29] (with parameters job=denovo,accurate,454,accurate,454
The shotgun sequence data were assembled using the TIGR assembler [144] and genome closure/finishing was performed using a combination of primer walking, PCR, and genomic DNA sequencing as in [145].
The sequence data were assembled using Roche's Newbler assembler, version 1.1.03.24, and open reading frames were identified using GLIMMER3 (Delcher et al. 1999) and the 'getorf' module of the EMBOSS package (Rice, Longden, and Bleasby 2000).
These data were assembled using Amplicon Express' proprietary in-house assembly pipeline.
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The data was assembled using a de novo assembly approach using both pcap [ 23] and newbler (Roche 454 software package) assembly algorithms to assemble the data.
High-throughput genome sequencing was performed using a HiSeq 2500 machine (Illumina, San Diego, CA), and the 100 bp short read paired-end data was assembled using the de novo assembly algorithm, Velvet (Zerbino and Birney 2008; version 1.2.08).
The resulting sequence data was assembled using the manufacturer supplied Newbler assembler followed by clustering using Phrap [38].
Quality-passed data was assembled using the de novo genome assembler AllpathsLG [ 57].
The metagenomic data was assembled using Newbler (version 2.0.01.14), which employs algorithm attempts to combine individual sequence reads into longer contigs.
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