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Expression of miRNA 27a-5p was quantified by RT-qPCR (miScript II RT and QuantiTect SYBR Green PCR, Qiagen) in a 7500 Fast Real-Time PCR (Life Technologies) and data were analyze with the ΔΔCT method.
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Data were analyzed with SPM12.
Data were analyzed with software Immunospot 5.0.32.
The data were analyzed with FlowJo v.10.
All data were analyzed with GraphPad Prism v7.02 software.
Mass spectrometry data were analyzed with MaxQuant software (version 1.1.1.25).
Quantitative data were analyzed with IBM SPSS19.0 software package92.
The data were analyzed with Students' t-test.
Data were analyzed with linear multilevel models.
Data were analyzed with specialized computer software.
Data were analyzed with Cox regression analysis.
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