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Analysis of ELISA data was performed with Microsoft Excel (Redmond, WA, USA).

Statistical analysis of the data was performed with computer software.

All statistical testing on animal data was performed with GraphPad Prism v7.0.

Analysis of UHPLC-MS/MS data was performed with the MultiQuant 2.1.1 software (ABSciex, Darmstadt, Germany).

All primary analysis of sequence data was performed with the Bowtie2, TopHat2, and Cufflinks packages31,32,33.

Statistical evaluation of data was performed with analysis of variance test and Student's t test.

The acquisition of raw data was performed with ProteinLynx Global Server v.2.2 software (Waters).

Subsequent statistical testing on regional data was performed with SPSS.

Hierarchical clustering of data was performed with Cluster 3.0 [3.0.

All of the scientific data was performed with high technical, scientific, and ethical standards.

Statistic analysis of lifespan data was performed with the log-rank test using SPSS 16.0.

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