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Analysis of clinical data was performed by J.R., Y.L., B.Y., H.W.W. and S.Y.

The bioinformatics analysis of ChIP-seq data was performed by Shanghai Jiayin Biotechnology Co., Ltd.

Visualization of read count data was performed by converting raw bam files to bigwig files using IGV tools60 and normalized to 1 million reads.

Statistical analysis of the data was performed by using a PC program (SPSS 14 for Windows).

The offline processing and analysis of extracted data was performed by Matlab scripts.

Regression analysis of XRF data was performed by means of the Minitab statistical software package.

The frequency analysis of the climatic data was performed by OriginPro V9 (OriginLab Corporation 2012).

Interpretation of histologic data was performed by one of two ocular pathologists.

Assessment for eligibility and extraction of data was performed by two independent investigators.

All statistical analysis of data was performed by using StatView software.

Collection of ED overcrowding data was performed by the attending EP, ED nurse and researchers.

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