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The aim to compare the primary outcome between treatment groups in an overall synthesis of available data was not achieved.
Complete agreement between experimental and computational data was not achieved until a negative regulator was placed as an intermediary between the CS and Hsf1/Skn7 (see Fig. 1 and the following subsection).
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By this method, evidence was obtained for the contention that PRN production loci were most prevalent in grasslands, however, robust quantitative data were not achieved.
Although, using step gradient mode to separate the isoflavones, the calculated and experimented data were not achieved very good agreement, we could estimate the closed retention time before experiment.
First, the short timeline for recruitment and data collection meant that data saturation was not achieved.
However, significant data reduction was not achieved, as it needed 14 parameters to explain 71% of both the temporal and spatial changes in water quality.
At the central and regional level the authors had the option of recruiting more participants if data saturation was not achieved.
Based on prior studies [ 26, 27] and consistent with the study design, we anticipated that we would need between 20 and 25 participants but planned to recruit more if data saturation was not achieved.
Despite rigorous checks with the implementation of this algorithm as published, and variation of parameters and similar experiments to reproduce the published data, this was not achieved and the implementation here consistently defined a larger number of putative sites.
Differences in genome size among families considering the newly established circumscriptions (i.e. Scrophulariaceae s.s., Orobanchaceae and Plantaginaceae) were assessed using a non-parametric Kruskal Wallis one-way analysis of variance (ANOVA) on ranks (normal distribution data and homoscedasticity was not achieved even after data transformation).
Spearman's correlation was used for the non-normally distributed V240EM data because a normal distribution was not achieved by transforming the data.
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