Flow data are computed with a polycrystalline, microstructure-based model which accounts for grain boundary sliding, stress-induced diffusion, and dislocation creep.
Data were computed with Astra software 6.1 (Wyatt Technology).
Data were computed with the Statistical Program for Social Science SPSSS, Chicago, IL, USA).
Data were computed with the ΔΔCt method (Livak and Schmittgen, 2001).
Maximum likelihood phylogenies based on protein data were computed with PHYML (Guindon et al. 2005) with 100 bootstrap replicates.
For qRT-PCR validation, gene expression data were computed with the ΔΔCq method, using three housekeeping genes: RPL30, β-ACT and GAPDH.
Comparisons of socio-demographic and perinatal data were computed with Student's t-test, the chi-squared test or the Fisher's exact test.
DNA location data were computed with information extracted from The Ensembl Genome Database (http://www.ensembl.org), NCBI Map Viewer (http://www.ncbi.nlm.nih.gov/mapview/) and NCBI Unigene (http://www.ncbi.nlm.nih.gov/sites/entrez?db=unigene) websites.
Multiple sequence alignments based on transcriptomic data were computed with program MUSCLE [ 120] (version 3.52) using species-specific synthetic sequences constructed by the concatenation of the consensus sequences of the major toxin families shared by all snake venom gland transcriptomes, i.e. BPP, LAO, PLA2, SVMP, and SP, in this order.
A completeness index of follow-up data was computed for each team with potentially eligible patients (14).
