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The Illumina data was assembled with the Velvet assembler (version 0.7.55; [ 45]) with a hash length of 61 and the following options; -ins_length 250 -scaffolding no -exp_cov 16 -cov_cutoff 5, to produce an assembly with a final graph with 51765 nodes, n50 of 2413, max 22432, total 37740533, using 73178660/94020728 reads.
The data was assembled using a de novo assembly approach using both pcap [ 23] and newbler (Roche 454 software package) assembly algorithms to assemble the data.
High-throughput genome sequencing was performed using a HiSeq 2500 machine (Illumina, San Diego, CA), and the 100 bp short read paired-end data was assembled using the de novo assembly algorithm, Velvet (Zerbino and Birney 2008; version 1.2.08).
The resulting sequence data was assembled using the manufacturer supplied Newbler assembler followed by clustering using Phrap [38].
Quality-passed data was assembled using the de novo genome assembler AllpathsLG [ 57].
The RNA-seq data was assembled de novo with the Trinity assembler to create a reference gene set to which the reads were mapped in order to obtain differentially expressed genes (DEGs) between the drought and WW conditions.
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Sequence data were assembled using NewBler assembler and Consed.
The GS FLX sequence data were assembled using Newbler assembly software.
These data were assembled using Amplicon Express' proprietary in-house assembly pipeline.
Sequence data were assembled using the PHRAP program (University of Washington, Seattle, WA, USA).
Mapped RNA-seq data were assembled with Cufflinks v2.2.0100 using Physcomitrella patens genome version 1.6.
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