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A third protein, ULBP-2, that was identified in all three secretomes but not substantially elevated in the microarray data was assayed as a control.
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Data were assayed by anova and an unpaired Student's t-test.
In all experiments, each data point was assayed in duplicate.
In all experiments, each data point was assayed in duplicate, with each individual experiment repeated three times.
To consider potential interactions between indoor stove/fireplace exposure and GST polymorphisms, we utilized genotyping data, which was assayed as follows.
The expression data obtained were assayed for consistency by performing ANOVA tests.
Inhibitors (initial concentration of 10 μM, 3-fold serial dilutions, 10 data points) were assayed in triplicate against all SFK constructs.
For evaluating the reproducibility with replicate data, two RNA samples were assayed in triplicate.
Samples were assayed in duplicate and data were averaged; assays typically contained 0.02 μg to 1.4 μg cell protein.
A number of other lipid metabolic genes, selected on the basis of the microarray data were also assayed by QPCR.
Gene expression data were previously assayed on the Affymetrix Mouse Gene 1.0 ST array and were obtained from GEO (accession no. GSE22297) (Aylor et al. 2011).
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