> -wrap-foot> Multivanalysesnalyses were conducted on data following realignment, but without normalization or smoothing, generating statistical maps in each participants' native space.
Preprocessing steps for fMRI data included realignment of the raw echo-planar images (EPI), slice-time correction, coregistration of the EPI images with the structural image, normalisation to the Montreal Neurological Institute MNII) template brain, smoothing with an 8 mm full-width at half-maximum Gaussian kernel and filtering with a high-pass filter (see 'Materials and methods').
The realignment data were not used in the subsequent GLM analysis, but manual inspection of the motion plots did not indicate stimulus-correlated motion (data not shown).
Following realignment, data were then smoothed using a Gaussian filter (full-width at half-maximum, 7.2 mm) to improve the signal-to-noise characteristics of the images.
Data processing included realignment, unwarping, normalization to the Montreal Neurological Institute space using an EPI template (resampling voxel size: 3 × 3 × 3 mm), spatial smoothing (8 mm full-width at half-maximum Gaussian Filter), and high-pass filtering (1/120 Hz cutoff frequency).
Following realignment, data were then smoothed using a Gaussian filter (FWHM 2.354 & in-plane fMRI voxel size mm) to improve the signal to noise characteristics of the images [Bullmore et al., 1999].
Preprocessing of the data used SPM5 realignment, reslicing with sinc interpolation, normalization to the Montreal Neurological Institute coordinate system, and spatial smoothing with a 6-mm full-width-at-half-maximum isotropic Gaussian kernel and global scaling (26).
Our fitting uncertainty of Gaussian centers in three dimensions is less than 3 nm, while the alignment of the colors, shown for YFP-Spc42-mTurqouise2, is within 12 ± 6 nm in the mother-satellite axis, which serves as a color alignment control for our SIM data and our realignment method.
Additionally, individual transcript queries in Ensembl include strain variation data that contain realignments of the original strain specific raw reads from Celera (including D2) to the reference B6 sequence.
