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Hence, according to the present data, proteins of the angel, 3635 or nocturnin families may deserve the same putative function as yCCR4, i.e. deadenylation, or at least share common substrate binding and/or catalytic specificities (for instance preference for RNA and/or poly (A) tail).
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The data (protein levels of proteins 3 and 4) was sampled at 10-second intervals for a total of 30 data points.
PPI data on proteins of known function were also obtained as prey clones during the course of screening.
The family data, protein pair data and results of other analysis below were stored in a partially normalized MySQL database for future reference.
From the vast amount of PPI data, protein interaction networks (PINs) have been vigorously investigated.
Interactome analysis collects data of protein-protein interactions occurring in cells.
Direct, non-relative abundance data of proteins, allowing a comparison of different proteins within and between samples, are still difficult to obtain on a large scale.
We reconfirmed our result by considering structural data for edgetically perturbed proteins of our data set (table 1).
Sequence data of homologous proteins of C. merolae OREs were obtained from the Gclust database (Sato 2009, version 2010-05, data set Gclust2010e29b).
Our proteomic data identified 4031 proteins, of which 160 proteins were overexpressed and 5 proteins were downregulated.
Our data suggest that proteins of this family have a common evolutionary origin with glycosidases of the α-galactosidase superfamily.
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