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Our solution is based on the LONI Pipeline environment [3], [4], a graphical workflow environment for constructing and executing complex data processing protocols.
Standardized testing and data processing protocols have been developed to ensure long-term valid data collection methods.
The fifth component in particular, essential experimental and data processing protocols, concerns the discussion in this paper.
Figure 6 compares the distributions of the measurement errors for RNA-Seq and chips for different data processing protocols (line styles and shades).
In step 2, metadata are provided to describe the overall study, such as title, description, sample processing and data processing protocols, keywords, and experiment type (Fig. 1a).
The same crystal was used for all data sets and the resolution (sin θ/λ = 0.7 Å–1) and data processing protocols were identical.
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We propose and demonstrate a measurement and data processing protocol that reduces the time required to record entropy profiles from time scales of weeks to time scales of hours – without loss in accuracy.
The microarray data processing protocol is described in the Supplementary Materials and Methods.
The data processing protocol is of particular importance for reproducing the MS images shown in the publication.
Therefore, the reduced transcription of aas in only comparisons of the wild type with the engineered strains (i.e. B, C, and D) validates the data processing protocol developed for the RNA-seq analysis.
The deconvoluted spectra by our data processing protocol were introduced to the NIST MS Search 2.0 software for automatically searching compound information from the NIST 08 library and the author-constructed standard library.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com