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Data processing, normalization, and error modeling were performed using the Rosetta Resolver system (version 7.2; https://www.rosettacommons.org/software).org/software
SM wrote the core code for l1- l2 regularization and contributed with the development of MATLAB and R scripts for data processing, normalization analysis.
AB contributed with the development of MATLAB and R scripts for data processing, normalization and analysis, performed the t-test and supervised analysis, and visualized the results.
> -wrap-foot> This motivated us to develop MIRACLE, a comprehensive and user-friendly open-source web application providing an end-to-end solution covering experimental design, sample tracking, data processing, normalization, as well as visualization and statistical analysis of the results.
ChIP-seq data processing, normalization, and statistical methods are proposed in the method section; analysis of ChIP-seq data from breast cancer cell lines (MCF7 and its tamoxifen-resistant subline OHT-MCF7) before and after treatment with 17 β-estradial (E2), are presented in the result section, with conclusions at the end of the paper.
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Data processing included normalization and resulted on the normalized melting curves and the respective negative derivative of fluorescence over the temperature plots, using the LightCycler 480® gene scanning software.
For microarray data processing and normalization, microarray chips were scanned using a GenePix 4000B scanner (Axon Instruments, Union City, CA) and median spot intensities were generated using GenePix 5.0 (Axon Instruments, Union City, CA).
Later, another of the data processing steps, normalization, is conducted.
One important step in data processing is normalization.
RNA extraction, hybridization and initial data processing and normalization were performed as previously described [ 39].
Data processing and normalization were performed according to standard procedures using a LOWESS program.
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