According to EU data protection law, permission is needed for processing personal data, and they can only be processed for the purpose for which they were obtained.
In terms of different data tasks, types, and characteristics, the required learning techniques are different, even a machine learning methods base is needed for big data processing.
In order to illustrate the functionality of the package, AgiMicroRna includes a typical uRNAList object that contains a data example with all the information that is needed for the data pre-processing and subsequent differential expression analysis using the AgiMicroRna package.
It should be noted that the methodology of using the region in the X-ray spectrum resulting from scattering implies that only the spectrum's shape is needed for the data processing by PCA.
However an additional legal framework is needed for data collection, storage, processing, including linkage of screening, follow-up and cancer register allowing to run and to evaluate the programme [ 7].
Obviously more programming is needed for reading the data, outlier control, data transformation, and post-processing the results to calculate confidence intervals, equivalence limits and plotting the graphs.
We hypothesize that if all other factors (assay protocol, microarray platform, data pre-processing) were equal, fewer individuals would be needed for the same statistical power using inbred animals as opposed to unrelated human subjects, as genetic effects on gene expression will be removed in the inbred populations.
The increasing use of databases in applications beyond "behind-the-firewalls data processing" is creating a similar need for watermarking databases.
No ethical approval was needed for this study, as data acquisition followed Austrian federal law on data safety, and data pre-processing and analysing occurred anonymously.
