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Each data point was performed in triplicate.
Each data point was performed in triplicate and the assay was repeated twice.
Each experimental data point was performed at least in duplicate and the corresponding signals were averaged.
Each data point was performed in triplicate and fit in GraphPad Prism with a Michaelis Menten non-linear fit.
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Finally, assignment of each data point is performed in a manner identical to that of FCM.
For each drug treatment, a minimum of three independent replicate experiments with three intra-assay replicates per data point were performed.
Computer assisted multivariate analysis of the resulting high volume data (8000 data points) was performed.
A least-squares fit of a line to these binned data points was performed.
A robust linear regression fitting using bisquare weights for all data points, was performed for the scatter plot for each tissue.
Subsequently, an analysis of variance (ANOVA) using data from all experimental samples (four normalized log2-expression ratios per time point for a total of 20 data points) was performed for each element.
Statistical analysis of the experimental data points was performed by the ANOVA test, which was used to compare measured data using the SPSS 12.0 statistical software program (SPSS INC. Chicago, USA).
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