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The amino acid alignments obtained were further refined using T-Coffee[20] to incorporate protein structure data on the alignment.
Given the dearth of empirically-derived data on the alignment behavior of peptides that could even be used to model thresholds for SSMs, we were motivated to systematically study the actual alignment behavior of short protein fragments.
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This process was performed using a web interface tool described in the alignments procedures section of the methods, where the data on the alignments are reported and graphically displayed.
Based on the data provided on the alignment scorecard, different combinations of c eBLAT alignments can be chosen to generate an EvoPrint.
Although InN/STO shows many promising properties, there is a lack of experimental data on the interface band alignment parameters of the InN/STO heterojunction to date.
Past field studies have provided some data on the relationship between road alignment and safety, but the effects of differing combined alignments on either lateral acceleration or safety have not systematically examined.
Because the accuracy of alignment (and thus the gene quantification) depends heavily on the input data, the alignment algorithm chosen, and the alignment parameters used, the mapping results can vary substantially from case to case.
Finally, in our hands homology models based on KcsA developed with Alignment A satisfy the mutagenesis data on which Alignment B is based as do models using alignment B, and models using Alignment A are more consistent with mutagenesis studies of other groups [ 3, 13].
The resulting data matrix is largely based on the alignment produced by Pyron and Wiens [ 39] for their comprehensive analysis of amphibian sequence data (Dryad repository doi 10.5061/dryad.vdoi 10.5061/dryad
This suggests that relationships among basal lineages of Pancrustacea, based on the amino acid sequences of mt PCGs, are still quite unstable, and that the choice of one topology over another may be dependent on the efficiency of the algorithm to explore the likelihood space, as well as on the alignment, data and taxon choice, and the matrix of amino acid replacement.
Interactive html files for picking primers were produced from interface codes and multiple alignment data on the rice locus with a single-copy cDNA model.
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