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Data of replicate spots were combined while omitting outliers (>2 standard deviations).
In order to minimize this effect, all sequence data of replicate host-pollinator associations were removed for both congruence analyses.
The script provides a quality control report showing the numbers and percentages of spots discarded during the various steps of the data analysis pipeline as well as data of replicate spots with signal ratios >2-fold different from each other.
Provided sufficient numbers of replicate wells are analyzed, this well-to-well variation in droplet volume is likely to be captured within the precision data of replicate concentration measurements and was, therefore, not considered as a separate component in the top down uncertainty estimation.
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Quantification of the cytometry data of replicates revealed an expected concentration-dependent behavior and the decrease in the number of viable cells (An−/PI−) matched with MTT and Trypan blue results.
The figures present data of three replicate experiments at least.
Data of four replicate wells was combined for analysis.
From the data of each replicate, all four genetic architectures were created.
The dye-swap signal ratios were inverted before the data of the replicate were combined.
To ensure that the data of each replicate are sufficiently reliable, t-tests were performed with MeV [ 79].
Combined data of two replicates was assessed by the Cox proportional hazards survival model.
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