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In 2007, for the first time it was possible to gather data on mating because of a prolonged drought involving South Madagascar.
While it may be argued that identification of species using mtCO1 is open to debate, all available biological data in the form of mating studies [17] [21] support the proposition of Dinsdale et al. [10].
Our data suggest that cessation of mating flights is not triggered by the act of mating alone, and is supported by previous studies [ 30, 31].
JMATING is the first complete and versatile software for the analyses of mating frequency data.
Our data indicate that the costs of mating in both sex functions are high in L. stagnalis.
JMATING is an easy-to-use program developed in Java for the analysis of mating frequency data to study sexual selection and sexual isolation effects from laboratory experiments as well as descriptive studies accomplished in the wild.
Determining the effect of this indirect benefit on the evolution of female mate preferences (or resistance) will require comparable data on the direct costs of mating with various males, and an understanding of how these costs and benefits integrate across generations and vary among environments.
Though the field and molecular data on mating frequencies were consistent, we noted that the molecular data yielded more sensitive measures on mating frequencies, allowing the detection of smaller differences in mating numbers than classical field observations.
Precopulatory isolation was determined from observations of mating behavior, and postcopulatory isolation, from progeny data.
These data are completely inconsistent with previous suggestions that the parasite expands asexually in the absence of mating.
These data answer the question of why peacocks have elaborate trains: elaborate trains increase their chances of mating.
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