The SGR database was developed to facilitate dissemination and data mining of extensive floral and fruit transcriptome data in the woodland strawberry.
For example, using small EST libraries to identify orthologous clusters of ESTs, Hughes et al. [ 20] constructed supermatrices with 71% missing data, and this was after exclusion of most of the data because of extensive paralogy.
The first and probably most important step of processing data consisted of extensive field observations during July, August and September 2011.
Data mining of extensive genome-scale databases, such as the Human Epigenome Atlas, ENCODE, Eukaryotic Promoter Database, DBTSS, database on CpG islands, FANTOM5, and many others, cannot be performed without efficient bioinformatic tools.
We combined the notification data with data of an extensive general practitioner (GP) network in the Netherlands (Integrated Primary Care Information (IPCI) database [ 20, 21]) employing a matched case-control design.
The study design, methods, response and quality of the data of this extensive second Dutch National Survey have been published elsewhere in more detail [ 22- 24].
The main strengths of our study include its nationally representative sample and use of rigorous study methods to collect the data and the availability of extensive data on confounders (NCHS 2010a, 2010b).
This makes analysis of transcriptome data complex because of extensive background noise.
TJJ contributed to the design of the study, acquisition of data, and extensive interpretation of data.
JAH conceived the study, secured funding, contributed to the design and coordination of the study, and participated in interpretation of data and extensive preparation of the final manuscript.
