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Analytical data of coprogen.
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"Data, data, data," added Mr. Gillis of BGC Partners.
PssA is involved in the production of coprogen that still can be produced by the improved β-lactam producing strains (Samol, unpublished data).
For correlation of coprogen to the fungal biomass, mycelia from the cultures were dried for 48 hr at 37°C and balanced and coprogen concentrations per gram dried mycelium were calculated.
All complemented mutants behaved similar to the wild-type with production of only marginal amounts of coprogen and terrein in the presence of iron.
In contrast, the Δ sreA mutant produced significantly higher amounts of coprogen in the presence of iron whereas terrein production did not significantly increase.
The gradient started with 10% B and reached 30% B after 4 min, increased to 55% B within 10 min and reached 100% B after 2 min, where it was retained for an additional 4 min. Quantification of coprogen was performed from a calibration curve of known coprogen concentrations.
Isolation of coprogen for generation of the calibration curve was performed by semi-preparative HPLC from culture supernatants of the Δ akuB and Δ akuBΔ terA strains and fractions were collected by automatic fraction collection.
Holes of 8 mm diameter were punched into plates and inoculated with 100 µl methanolic solutions of coprogen, terrein, or propylene maleic acid in concentrations of 2.50, 1.25, 0.625, and 0.3125 mg/ml.
The data of Figs.
Inactivation data of the sustained current (s.c).
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