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The data obtained were averaged by software (Mastersizer 2000).
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LOS data so obtained were averaged between these 10 runs.
The data obtained were the average of three independent determinations.
The rotation rate of the cells was measured by detecting the position of the cell every 50 ms. The data obtained were smoothed (100 points), averaged (for as many cells as available - at least 20 per graph) and plotted.
The data obtained were normalised using the geometric average of the 2−ΔCq values of three stable reference genes selected out of a set of 10 [ 83] by geNorm (v3.5) and Normfinder (v0.953) algorithms [ 84],[ 85].
The data obtained was evaluated based on average contig length, N50 values, and percentage of reads assembled (Additional file 2).
The absorbance was measured at 570 nm using a microplate reader (Model EL 800X), with 630 nm as reference wavelength and the obtained data were averaged and fitted to Eq. 1, to determine the percentage of cell viability.
The normalised data obtained from duplicated hybridisations were averaged to obtain a final data set.
To obtain means for subsequent analysis, 0 30 min data were averaged to obtain steady-state baseline values, and for comparisons against baseline, 40 90 min data were averaged to obtain steady-state infusion period values, and 100 120 min data were averaged to obtain steady-state recovery period values.
The measured data were averaged to obtain the specific heat and rheological properties of the NCBNF.
The data obtained from ten consecutive plants were averaged to obtain trait values for each plot, and three replications were averaged to obtain trait values for each line in each experiment.
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