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Overexpression of TXNIP1 did not alter the response of astrocytes to H 2O 2 whereas co-culture of neurons with astrocytes depleted of TXNIP1 did not result in enhanced neuronal survival (data not visualized for clarity), indicating that this route is involved in the response of astrocytes to oxidative stress but not in neuroprotection.
When using this ROS marker it is not possible to distinguish endogenous ROS from exogenously applied H 2O 2. Nevertheless, we compared this method to the oxidation of luminol (which detects superoxide anions) that distinguishes H 2O 2 from other ROS and we obtained identical results with either method (data not visualized).
Similar(58)
Patients were excluded from analyses if the pancreas was not visualized on ultrasound or laboratory data was incomplete.
Normal ureters are not visualized on ultrasound.
We had not visualized these".
As expected, normal mitotic cell configurations in the different mitosis stages were visualized (data not shown).
About 300 spots in each Sypro Ruby-stained gel were visualized (data not shown).
The transfection efficiencies were estimated by visualizing DsRed fluorescence (data not shown) or by immunocytochemistry of TAS1R3, and were typically 50∼55% (Figure S4).
All cells were incubated with the DNA fluorescent dye Hoechst 33342 (Invitrogen) to visualize their nuclei (data not shown).
Under the endogenous promoter, there was not sufficient signal to visualize the protein (data not shown).
Indeed, the SpDre4-TAP complex was not abundant enough to be visualized following sucrose gradient sedimentation (data not shown).
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