These results confirmed the RNA data obtained in the screening performed using the cDNA arrays.
All participants with complete data recorded in the screening register were included in this analysis.
In addition, the demographic data reported in the screening phase will be analysed to compare those who responded to the general population.
These results confirmed the RNA data obtained in the screening performed using the microarrays for all the selected genes with the exception of Grb2.
Because of incomplete data in the Psychosocial Screen for Cancer, another 220 individuals were excluded from analyses resulting in a final sample of 1,646.
Of the one-to-one orthologs, 7,939 (from 13,784 lines) have knock-down data in the Bristle Screen database, with 929 (~11%) genes (from 1,291 lines) being completely lethal.
Of the 15,379 Drosophila reference genes present in our modules, 10,753 were included in the 18,663 lines with knock-down data in the Bristle Screen database, with 1,237 genes (in 1,655 lines) showing completely lethality (~8.868%).
Among the orphan genes, 52 (from 84 lines) have knock-down data in the Bristle Screen database, with only 1 gene (from 1 line) being completely lethal (Additional file 15).
The involvement of the TOR pathway in heat-stress resistance/sensitivity has been described (Bandhakavi et al. 2008) and is highlighted by the data in the present screen because the rim15 Δ mutant was sensitive in both exponential and stationary phase and tco89 Δ was resistant in stationary phase cells.
The drug likeness of each scaffold was evaluated using the ratio of the hit rates in drug data set to that in the screening data set (a subset of the ZINC database containing 1.95 million entries) [21].
