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To control for this variability, the data in each experiment are interpreted as a relative level to the control condition where cells were electroported with the inert RNA encoding GFP.
All data in each experiment were fitted simultaneously.
Frequency distributions of colonisation data in each experiment were multi-modal and suggest that several QTL are involved in controlling this trait (Additional File 3).
Even though it is not feasible for us to perform a comprehensive evaluation of the quality of the data in each experiment, a meta-analysis is in its essence a technique to eliminate poor quality data and hence we are confident that there are no systematic errors in our analysis that artificially originate false results.
The reliability and reproducibility of datasets was ensured by the use of duplicate or triplicate hybridization data in each experiment, normalization of all arrays to the median probe intensity and the use of normalized CEL intensities of all arrays for the calculation of model-based gene-expression values based on the Perfect Match-only model [ 42, 43].
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At least three samples with paired data were used for data analysis in each experiment.
Due to the large amount of data collected in each experiment, a semiautomatic data analysis tool was written in Matlab (The MathWorks, Natick, MA), which analyzes data sets to extract relevant information.
Data from studies with multiple experiments and one sample per data point in each experiment (Western blot experiments) were expressed as mean±s.d.d
Data is from 2 independent experiments and 200-300 cellscoreded for each data point in each experiment.
This work was also expanded (O'Keeffe & Cinnéide, 2008b) with a larger set of input programs, greater number of data points in each experiment and more detailed discussion of results and conclusions.
When the training and testing data sets in each experiment are identical with respect to the native origin of the speakers, the accuracies are higher compared to the cases where training and test sets are different with respect to the native origin of the speakers.
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