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Following cleaning of data, high quality reads were obtained for control and Cd-treated leaf and root samples (Additional file 2: Table S2).
However, in order to systematically explore relationships and breeding patterns from wild specimens using whole genome sequencing data, high quality DNA is required and in most field studies, non-invasive samples such as feces or hair are used, and the amount of DNA extracted from such samples precludes the application of this methodology to conservation studies.
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To extract quantitative information from digital SWATH-MS data, high-quality assay libraries are required.
By later removing reads containing adapters, reads containing poly-N and low-quality reads from the raw data, high-quality read pairs were obtained.
While our study is based on population-level data from high quality data collections, there are a number of limitations.
Furthermore, SEER data are high quality and are collected in a uniform manner with uniform data standards.
Although data of high quality are collected, we have been unable to access reliable aggregated data for these countries.
Therefore, the GBS data have high quality SNP calls for linkage and QTL mapping.
All the downstream analyses were based on the clean data with high quality.
All downstream analyses were based on the clean data with high quality.
These tests were highly successful and the data of high quality.
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