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Data from the scoring assessment of p53 on the TMAs were entered into, the data handling program (Cansto) at the Garvan Institute of Medical Research, Sydney.
Disaggregated cell suspensions were fixed with 75% ice-cold ethanol overnight and then incubated with 10 μg/ml propidium iodide (Sigma) in the presence of 500 μg/ml RNase (Sigma) for 60 min. Cell cycle data were collected on BD FACSCalibur Flow Cytometer (San Jose, CA) and analyzed using CellQuest data handling program.
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A data scientist is one who combines sound business understanding, data handling, programming, and data visualization skills to maximize business impact by working with data mining making sense of it, building statistical models, and proving causality to answer questions which drive business forward.
Most data handling programs require specific save commands in order to preserve any edits that a user does while in the system.
All quantification was done by a built-in data-handling program provided by the manufacturer of the gas chromatograph (Perkin-Elmer, Norwalk, CT, USA).
All the sequence analyses and data handling were done using programs written in the Python programming language (ver. 2.6, http://www.python.org/).org/
The specific processes for data handling should be customized by programming both the interaction with end-users' dialogs and with the database management system.
A further important aspect in the transition of epidemiology is that programming and data handling skills have become more essential, in particular when one needs to deviate from standard analysis.
DC wrote basic computer programs for data handling and participated in the analysis.
We also highly appreciate the contribution from Elin Bergseth at NORSVIN/BioBank AS for collection and handling of samples, Thomas Moen, Harald Grove and Torfinn Nome for helping out with programs for data handling, and Martien Groenen for kindly providing the physical SNP positions of the 60 K Porcine SNP array.
All data handling was performed using Chipster, a visual program based on R Project for Statistical Computing program (Center of Scientific Calculating (CSC), Finland).
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