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Data from the temperature telemeters were recorded at 500 Hz, for one second, once per minute for 23 hours using Data Sciences Int. acquisition software.
Correlation analyses of the individual RT-qPCR transcript data from the temperature and low oxygen stress experiments are shown in Additional file 5. From the temperature stress experiment, many of the evaluated target gene transcripts were strongly correlated with each other (Additional file 5, worksheet A).
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To investigate the effect of temperature on the ingestion of defended prey, we initially pooled the data from across the temperature manipulation phase and calculated the mean number of defended prey eaten at each temperature for each bird.
Potential evapotranspiration data was extended using the Thornthwaite equation, based on data from the Central England Temperature series and potential evapotranspiration from the Met Office Rainfall and Evapotranspiration Calculation System.
The data from the two temperature conditions were assembled separately.
In all cases, the data from the elevated-temperature response curves were used for analysis.
It has to be stressed though, that the data from the high temperature-treated cross was not as informative as that from family 1 or family 2. The fact that the high temperature-treated group contained almost all male progeny, with only 4 females, forced us to analyse both treated and control groups simultaneously, adding the factor treatment as a fixed effect (treated; untreated).
It takes decades of data from the US Geological Survey on temperature and precipitation across the US, and insures weather-dependent businesses (farmers, housepainters, golf ranges – anyone involved in the outdoors).
Data from the accelerometer and temperature loggers were downloaded onto a PC using custom-made software.
The mean percentage deviation between the field temperature data and the temperature data from regression relations is estimated by Eq. 3.
An empirically-based constitutive relation was extracted from the data spanning the temperature range 24 128°C.
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