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This concept is largely based on data from the fly wing epithelium demonstrating that PCP proteins restrict the formation and localization actin-rich structures.
We obtained the paired end sequence data from the fly developmental modENCODE project [ 22] from the European Nucleotide Archive [ 52] and calculated expression levels of isoforms from FlyBase 5.45 for all developmental stages.
We obtained the paired end sequence data from the fly developmental modENCODE project and calculated expression levels of isoforms from FlyBase 5.45 for all developmental stages using the same protocol as used in the original modENCODE analysis (see Methods).
To investigate the influence of the epigenetic contexts on chromatin dynamics, we generated and used Hi-C data from the fly Drosophila melanogaster for which epigenetic domains have been extensively described [ 6].
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We went on to analyze how far the data obtained from the fly model can predict the outcome of the murine model experiments.
As a final and biologically relevant measure, we wanted to investigate whether virulence data obtained from the fly model is able to predict the overall virulence phenotype in mice.
More importantly, a comparison of our data with microarray data from the tko fly line with a mutation in the mitochondrial protein S12 [38] revealed that a subset (∼15%) of genes affected in the U6atac mutant are also affected in the tko line despite the differences in developmental stage (adult vs. larvae) or different microarray platform used.
Data from the fruit fly have already suggested a role for Neph proteins in cell migration and fusion (Ruiz-Gómez et al. 2000; Strunkelnberg et al. 2001).
This toolkit, allowing users to interact with 2D GIS data on the Internet and create perspective views from these data on the fly, illustrates how to extend an existing Internet GIS into a more powerful virtual GIS.
It is intended to assist the user to extract subsets of data on the fly directly from the respective resources, avoiding duplication of data on local machines.
Federated approaches, such as the distributed annotation system (DAS) (Prlić et al., 2007) establish a central view on the data by translating each internal query into a set of external queries in order to retrieve the required data from the outside sources on the fly (Heimbigner and McLeod, 1985).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com