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Data from separate experiments were pooled when homoscedasticity was significantly tested with the Bartlett test.
Student's t-test or one-way anova was used for comparing mean data from separate experiments.
These results suggest careful consideration should be given to using animals in fresh versus conditioned media for experimentation and that composition changes must be taken into account when comparing data from separate experiments.
The unique feature of our experimental design that enables us to compare data from separate experiments is that identical replicate reference samples of pooled RNA (that are derived from the same tissue as are the experimental samples) are included in each batch within each experiment.
Since the RNA samples of body parts originated from the same batches of flies, our approach eliminates any biases that might be encountered when comparing microarray data from separate experiments (differences in rearing conditions or age, in genetic background or in extraction methods).
Similar(55)
The patterns of cytokine expression detected by ELISA (Figure 2) and Luminex (Table 1) were in good agreement although the magnitude of the cytokine responses cannot be directly compared since the data are from separate experiments.
The qRT-PCR was performed in triplicates and data were expressed as the mean ± SD from separate experiments.
Data are expressed as mean ± standard error of the mean of triplicate values from separate experiments.
For each pathogen, 3 6 successive time periods were studied and for each time period, data from 2 4 separate experiments were obtained and, using the data from these multiple experiments, 2-way ANOVA analysis were carried out.
The data are plotted as the average data from two separate experiments.
The data from 5 separate experiments are shown in Table 1.
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