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This is especially important when considering very low-level heteroplasmy detection in MPS-based datasets, since comparative data from methodologies that have been fully validated for forensic use (i.e. Sanger sequencing) will be unavailable.
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Applying this method is consistent with the use of varied data from diverse methodologies [ 66].
The CPGR is already incorporating data from these methodologies and merging them with data generated from 'first generation sequencing platforms'.
See Also: Where Does the Data Come From (Methodology)?
Another explanation may invoke inconsistencies due to the collation of data from widely different methodologies.
Studies have used data from various other methodologies for similar exploration such as data from national surveys (32), a LIST model exploration (33, 34), and a health system evaluation framework developed by Countdown In-Depth Country Case Studies (34).
However, regardless of the methodology used for SNP discovery in tall fescue, the haplotype complexities created by the allohexaploid genome structure will still need to be addressed, and the approach taken in this study is applicable to data from multiple sequencing methodologies.
The observation of neuropsychological effects is also limited by several factors including absence of an appropriate control group, the small number of cases, lack of baseline outcome measures for IQ testing, use of non-validated comparison data from different test methodologies between evaluations, and significant delay between exposure and evaluation.
Data from New Approach Methodology(ies) including high throughput screening, in vitro and in chemico assay and in silico tools, may provide critical information needed to strengthen the toxicodynamic similarity rationale.
Confidence in the usefulness of the methodology can be improved if the spatial and temporal patterns of bicoherence can be correlated with simultaneously acquired data from a complementary methodology like functional magnetic resonance imaging.
As a result, our review is based on data from one particular methodology.
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