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Since a minimum of 8 samples/group is recommended [ 27], expression data from different organs were also combined into "vegetative" (roots and leaves; n = 12) and "reproductive" (flowers and fruits; n = 15) sample-groups.
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This set of genic-SSR markers will be valuable for genetic research in sesame on aspects such as growth and development processes or biotic stress traits, since our transcriptome data was derived from different organs, developmental stages, and stress treatments.
Cacao transcriptomes: The Esttik database (http://esttik.cirad.fr/) houses EST discovery data from fifty-six different cDNA libraries constructed from different organs, genotypes and in varying environmental conditions [ 37]. 454 transcriptome: A cDNA library from six mixed tissues of Criollo genotype (B97-61/B2) wasequenceded by Roche/454 technology as previously described [ 36].
Similar data processing was performed with the microarray results from different organs.
Moreover, several positive specimens from different organs in pigs and placentae in sheep (unpublished data) as well as conjunctival and nasal swabs from cats [ 34] have been demonstrated by the same PCR at our laboratory.
Figure 3 Histology of tissue collected from different organs.
Also, expression was compared with corresponding DC subsets from different organs from mice.
Total RNA was isolated from different organs, as described by Wang and Vodkin (1994).
Stop names are not standardized: two otherwise identical stops from different organs may have different names.
Tissues collected from different organs were homogenized in 1 ml of ice-cold DMEM.
To identify whether this is a common phenomenon, cells originated from different organs were used.
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