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We further used long-read and short-read datasets from both real-life and in silico NGS data for comparative analysis and evaluation of these aligners focusing on three criteria, namely, application-specific alignment feature, computational performance, and alignment accuracy.
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Real data is indispensable for comparative analysis and for the development and improvement of preprocessing tools [ 53, 58].
The number of individually florescent spot as test cells was then scored for comparative analysis and the data was exported to Microsoft Excel.
Data for comparative analysis of the body proportions and the locomotion were taken from [33].
The uORFSCAN pipeline used rice full-length cDNAs [ 39] and wheat, barley, maize, and sorghum assembled EST data for comparative analysis.
While the last might appear the biggest problem, we found that ab initio predictions combined with manual curation and interspecies comparison have proven to be very accurate (e.g. see PKD1L example), thanks to the compactness of the Fugu genome, smaller ratio between intron and intergenic region sizes compared with mammalian genes, wealth of data for comparative analysis etc.
The main functionalities of varDB include querying and retrieving antigenic variation sequence data for comparative analysis.
Data for comparative analysis of gene expression were obtained using the ΔΔCt method (Livak and Schmittgen, 2001).
Another limitation is the small population in some groups especially those with abnormalities on imaging studies, chronic diarrhea, and chronic abdominal pain, which do not provide adequate data for comparative analysis.
Complementary components of the data set included categorical and numeric data for quantitative comparative analysis and narrative data for qualitative thematic analysis and elaboration.
Data from 2005 2007, the "pre-IMSD era," represent baseline data used for comparative analysis.
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