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Our data suggests that for species unrelated to Arabidopsis it would be better to use both introns predicted and empirical data for assay design to maximize validation rate and evaluate genetic diversity.
Detailed analysis of the screening data for assay #30 showed that 14 pools that should give a signal for BnaC.WRI1.b were only assigned to the category specific for BnaA.WRI1.b.
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Similarly, the data for assays #11392a and #386-14d in this study had also revealed that individual loci had escaped detection in the library screen.
Data for assays including mutagenicity in Salmonella and mouse lymphoma cells, chromosomal aberrations, and sister chromatid exchange in Chinese hamster ovary cells, transformation of Balb/c 3T3 cells, and in vivo cytogenetic effects in rodents have been compiled for many chemicals.
As has already been indicated in the immediately preceding Section 3.2, this approach could be adopted within the present IEDB framework by specifying the conditions as data embedded in the existing data field for assay comments, albeit without the benefit of data standardization that otherwise could be achieved by introducing additional data fields.
Nevertheless, any available data on the median effective dose could be embedded within the existing data field for assay comments, so as to facilitate future assembly of benchmark datasets from such maximally informative data.
Table S2 summarizes data for adhesion assay (a - d ), caspase 3/7 assay (e ), and proliferation assay (f ).
Summary findings with respect to other seminal characteristics are given in Tables 4 and 5 for the ER CALUX assays (data for other assays not shown).
Manual reference data for intra-assay and inter-assay coefficients of variance is 1.6 4.2 and 3.3 6.4 %, respectively.
All of the data for an assay can be represented as a table with one row per chemical and one column per assay component.
The final absorbance was noted up to 6 min and data for each assay was recorded in triplicate.
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