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Pre-acute lung injury (ALI) (Table S1 in additional data file) was tested only against baseline ALI (BL-ALI).
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The primers are listed in Additional data file 2. In addition, the expression of 180 adhesion, migration and cytoskeleton genes (see Additional data file 3) was tested from 1 μg cDNA using SYBR Green reagent on an ABI7700 system (Applied Biosystems, Foster City, CA, USA).
Pre-acute lung injury (ALI) (Table S2 in additional data file 1) was tested only against baseline ALI.
Following completion of the testing, the data file was sent electronically to the main coordinating centre for analysis using StataSE 9.2.
At the end of each test, the data file was exported and analyzed using MicroSoft Excel 2011, with the mean of the final 90 s of each 2 min period of steady-state used in the final analysis.
The α data file was subdivided into three subfiles: the learning file (ZU) containing 330 cases, the validation file (ZW) containing 110 cases and the test file (ZT) containing 110 cases.
The evaluated data file is being assembled and subjected to rigorous testing, verification and validation.
The data file is stored as a.TXT file and considered to be the training and testing data set (Table 2), which contain 17 samples in total, each sample has 34 variables.
The feasibility of the data extraction form (see Additional file 3, Word document: data extraction form) was tested with a sample of three articles eligible for this review.
First, data was tested for normal distribution.
The data files were also extensively tested using the statistical package R (see Methods) and were examined by cluster analysis and pair wise using scatterplots.
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