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To investigate the validity of array data, expression levels of the differentially expressed genes were measured using the TaqMan quantitative RT-PCR assay.
Data are expressed as mean ± standard deviation; different data expression is stated in the text.
Normalized expression data, expression cluster information and corresponding annotations are available for all differentially-expressed trout clones in a searchable excel spreadsheet provided in additional file 1.
Based on the MPSS data, expression of OsOXO4 was lower in the M. oryzae-Nipponbare (Pi-9) resistant interaction.
For non-UMI data, expression may be obtained as counts using tools such as HTSeq [49], RSEM [50], WemIQ [51], and featureCounts [52], among others.
Moreover, it is valuable to develop information mining techniques for dynamic wide-area data and investigate in-depth data processing and effective data expression.
Further, based on quantitative real-time polymerase chain reaction data, expression of inflammatory genes was upregulated for the 100- and 200-nm surfaces in macrophages and foam cells.
Note that if the target-like signals appear in the training samples or the clutter characteristics of the training samples are non-homogeneous with those of the CUT data, expression (13) will lead to an inaccurate estimation.
In addition, proteomic characterization as well as microarray data (expression of >22,000 genes) coupled with KEGG pathway analysis and gene set expression analysis (GSEA) supported our development of less-inflammatory 3D BMAT compared to 2D culture.
Consistent with these data, expression of Sox9, Sox10 and SNAIL was upregulated during neuralization of hESCs.
TRANSMODIS is a probabilistic model for predicting direct targets from binding motif, sequence data, expression data and ChIP-chip experiments.
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