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The known PPIs were downloaded from the IntAct [56], where the high-quality molecular interactions were collected from the curation of published literature or from the data depositions of the expert curators.
However, at this point, spectral data depositions in open exchange formats cannot be made mandatory, as some of the open exchange formats cannot handle complex multistage chromatographic data sets, such as LC-MS with MS2 and MS3 scans and ion trees.
This is expected to further increase the utility of open access data depositions.
Here, we describe the MetaboLights curation pipeline, its challenges and its practical application in quality control of complex data depositions.
Here, we describe the PRIDE curation pipeline and its practical application in quality control of complex data depositions.
While PRIDE faces rapid increases in data deposition size as well as number of depositions, the major challenge is to ensure a high quality of data depositions in the context of highly diverse proteomics work flows and data representations.
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Data deposition: All data sets have been deposited in the Gene Expression Omnibus, www.ncbi.nlm.nih.gov/geo (accession nos. GSE10041 and GSM253663-253734).
Data deposition: The Affymetrix microarray data have been deposited in the Gene Expression Omnibus (GEO) database (GEO accession no. GSE24271).
Data deposition: GeneChip raw data (CEL-files) have been deposited for public access in the Gene Expression Omnibus (GEO), www.ncbi.nlm.nih.gov/geo, accession number GSE18920.
Data deposition accession numbers: The microarray data have been deposited in NCBI's gene expression omnibus (GEO) accession number GSE34016 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi).nih.gov/geo/query/acc.cgi
Data deposition: The atomic coordinates and structure factors have been deposited in the Protein Data Bank, http://www.pdb.org/ (PDB ID code: 3N2N).
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