LP, GdG, AP have conceived the study, have selected and developed the assessment instruments, have organized and supervised data collection, were actively involved in data analyses and have drafted the paper.
Funding was provided by the Joint Fund South China Botanical Garden-Shanghai InstitutEcology, National Natural Science Foundationtural Science Fofndation of China (31200176, 31200246, 30900089) and Key Laboratory of Plant Resources Conservation and Sustainable Utilization, SCBG, CAS. Isolation of the sequences and real-time PCR analyses and data collection were accomplished by Q.F.
Researchers participating in the study received training for data collection standardisation and collected data were analysed centrally.
Longitudinal qualitative data collection will be analysed between data collection points, to build our theory of how the intervention is working or not working.
This was followed by preliminary analysis of data conducted by two of the authors, who jointly determined the point in the fieldwork process when little new information was produced, after which data collection was concluded.> Data was analysed through multiple readings of the material.
In addition, the process of data collection was conducted, coded and analysed in Swedish.
After data collection was completed, three researchers independently analysed all 100 consultation transcripts (including those 25 analysed previously) in NVivo, in relation to the checklist.
Applied Biosystems 7300 Real Time PCR instrumentwas used for data collection, and the resulting data were analysed by the 2 ∆∆Ct method to obtain fold difference in expression between genes of interest compared to optimal (LDSN) condition [ 56].
Data were extracted using a standardised data collection form, and were analysed independently by three reviewers.
Record forms and a semi-structured questionnaire were used for data collection, which was analysed with Stata 11.0 software.
