Data for 10 cells were collected for each sample and prior to data collection cell debris was excluded by setting a gate on a forward vs side scatter two-dimensional dot plot.
Data collection, cell refinement and data reduction were performed with SMART (Bruker, 1998), SAINT Plus (Bruker, 1998) [31] respectively.
Data collection: The cells are monitored by means of different metrics, such as configuration management (CM) parameters, performance management (PM) parameters, performance indicators (PI), and mobile traces.
In particular, the framework proposed in this paper follows the scheme shown in Fig. 2. Data collection: The cells are monitored by means of different metrics, such as configuration management (CM) parameters, performance management (PM) parameters, performance indicators (PI), and mobile traces.
The programs used for the crystal-structure determination are – Data collection : APEX2 [26], Cell refinement : APEX2 and SAINT [26]; data reduction : SAINT and XPREP [26]; structure solving : SIR92 [27] ; structure refinement : SHELXL97 [28] ; molecular graphics: ORTEP [29] and Mercury [30].
Data collection and cell quantification was performed in a blinded fashion.
This article can be improved by clarifying the following minor points in the section Data collection and cell-type classification: 1. Cluster quality It is not clear whether the quality of single units was assessed objectively, for example, by measuring isolation distance.
Cell determination, data collection, data reduction, cell refinement and absorption correction: CrystalClear-SM Expert 2.0 r7.
For example, data may fluctuate depending on cell type, timing of data collection (during the cell cycle, or diurnal, seasonal, or annual variations), developmental stage, and various external conditions.
NF023 (50 µM), D609 (50 µM), U73122 (12.5 µM), and anti-STC1 (1 µg/mL) was added directly to the 200 µL of Fluo-4 assay buffer containing Fluo-4, and incubated alongside the Fluo-4 for 30 min prior to data collection as the cells took up the dye.
