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Stock solutions were then diluted in buffer to 10 μM for data collection and determination of the thermodynamic parameters for siRNA unfolding.
Our study highlights the importance of standardisation of the methods used for storage and staining of tumour tissue as well as the patient population, data collection and determination of clinical end points.
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Here we go beyond the narrow documentation of final crystallization conditions that produced the refinement-data crystal, to describe the pathway to successful crystallization, data collection and phase determination of this difficult protein with the hope that our experiences will be instructive to others faced with challenging crystallization problems.
Sue Roberts and Andrzej Weichsel provided guidance in the MAD and native data collection and structure determination.
Data were collected on the ID23-1, ID23-2, or ID29 beamlines at the European Synchrotron Radiation Facility (Grenoble, France) and processed as described in Supplemental Material, "Data collection and structure determination".
Crystallization, Data Collection, and Structure Determination The 7c12 monobody as isolated from a phage-display library was not sufficiently soluble for crystallization trials.
Data collection and structure determination: Complete data sets for the wild-type protein (1.15 Å resolution) and the FDC_Ec E72A variant (1.50 Å resolution) were collected at the beamline BM30A[ 37] and ID29[ 38] at the ESRF Grenoble, France.
X-ray diffraction data were collected at the Advanced Photon Source beamline 24 ID-E (Argonne National Laboratory) at a wavelength of 0.97917 Å and a temperature of 100 K. Data collection and structure determination statistics are given in Table S1.
5 This approach is based on the rapid generation of protein ligand complexes through the soaking of protein crystals with ligands and subsequent automated X-ray data collection and structure determination.
Clinical data collection and biomarker variable determination were performed in 132 subjects.
Clinical data collection and biomarker variable determination were performed in 1,828 subjects.
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