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So the D2 data cannot be fitted with the transfer matrix method directly.
Because most experimental data cannot be fitted with a single analytical function for all τ values, we have developed a method to fit the MSD at consecutive intervals in a splinelike manner [41].
In fact, experimental data cannot be fitted to a linear buffer curve [ 4].
Lack of fit can easily be flagged and if data cannot be fitted properly, results should be interpreted with care.
(2) Δ F = Δ F max peptide / K P + peptide We note that KL and KP are not equivalent (i.e., the FPE and Trp fluorescence data cannot be fitted to a single equilibrium binding constant) since the peptide membrane interaction corresponds to a partitioning rather than a discrete site-binding phenomenon, as described in more detail elsewhere [1].
aNo CI (for perfect discrimination, data cannot be fitted to a ROC curve) bValue interpolated from empirical data using Weibull cumulative discrimination function Assay sensitivity was markedly decreased by the use of LS_DEC or LS_ALL probe sets as analytes for detecting modeled changes of 1.5-, 2-, and 4-fold as a function of RIN level.
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In addition we ask the question: What if data cannot be fit using the fitting functions incorporated in the data-analysis software that came with your ITC? Examples where such data might be generated include systems following non 1 n binding patterns and systems where binding is coupled to other events such as ligand dissociation.
These data cannot be fit to a single-site binding model as in previous work (9).
Also, these data cannot be fit to a two-site, sequential model.
Indeed, even if we were to assume that the signal change now should report on the formation of a singly ligated species, the data cannot be fit with a simple 1 1 binding model (solid line).
This may occur because a particular data set indeed cannot be fitted to a tree (and would be better represented by a network).
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