Exact(1)
To determine whether genes for csRNAs are present in other streptococcal species BLAST searches [ 39] of the nucleotide collection (nr/nt) data base were performed using the five pneumococcal csRNAs as query.
Similar(59)
Statistical comparisons of chip data based on ANOVA were performed using the software package JMP Genomics, version 4.0 from SAS SAS Institutee, Cary, NC, USA).
Primary data analysis and base calling were performed using the Illumina instrument software.
For the multiplexing sequencing, 100 cycles of single read 1 was used to sequence the RNA, followed by 7 cycles of index identification and 100 cycles of single read 2. Primary data analysis and base calling were performed using the Illumina instrument software (Illumina's Consensus Assessment of Sequence and Variation, CASAVA).
Base calling and FASTQ data conversion were performed using the Illumina pipeline CASAVA 1.8 package.
Rarefaction curves based on ARDRA data were performed using the independent sampling algorithm, implemented in the EcoSim program (Gotelli and Entsminger 2003).
Multivariate statistical analysis of hyperspectral data cubes were performed using NSS COMPASS, which is based upon research performed at Sandia National Laboratories [18, 19].
Image analysis and base calling of the raw sequencing data were performed using the Illumina data analysis pipeline v1.4.
The conclusive tree construction based on the NCBI taxonomy [47], visualisation and data analysis were performed using unpublished software.
Normalization based on the LOWESS algorithm [ 88] and data analysis were performed using Genespring software version 7.2 (Agilent Technologies, Santa Clara, CA).
Data analyses were performed using VMD56.
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