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In order to analyze the observed mRNA dynamics by objective means, we convert our schematic description into a rigorous mathematical model and use experimental data and statistical testing to quantitatively assess the amount of evidence for each alternative scenario.
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GraphPad Prism was used to graph the data and statistical tests used are located in the figure legends.
Topic covered in the first section include first-tier (minimum) and second-tier tests, sources of variability, analytical considerations, appropriate use of normative data and statistical tests, and evaluation of peripheral blood and bone marrow smears and histologic bone marrow specimens.
Computation of data and statistical tests were performed using the Statistical Package for Social Science software (SPSS).
Analysis of spike data and statistical tests were performed using MATLAB (MathWorks).
For a generalization, however, more data and statistical tests would be needed.
Following preprocessing of data and statistical tests on differential expression, significantly upregulated or downregulated probe sets (false discovery rate <5%) were identified and then mapped to Ensembl genes http://www.ensembl.org/index.html.html
The SPSS 20 software, Log Rank test, was used for data analysis and statistical testing as well as kaplan meier for survival studies.
All data analysis and statistical testing was conducted using the statistical programming language R [89].
Data plotting and statistical testing has been performed with the Origin 7 package.
If not noted otherwise, all data analysis and statistical testing was done in the statistical programming language R [57].
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