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Data collection and data analysis were executed iteratively.
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Data analysis was executed using Ultrascan II [34] where 3 scans at 3 different speeds were simultaneously fitted to a one-component model.
Data analysis was executed in analogy to Probe based Real-Time PCR.
Below we explain briefly how data collection and analysis were executed in this study.
Thus, the fitting analysis was executed with four different data sets.
A factorial map of principal component analysis was executed on the whole expressed data by the program Qlucore [ 68] on the differentially expressed (fold change >2).
Using this model, a thermal parametric analysis is executed and from the corresponding statistical analysis of the data generated, the practical expressions are derived.
In terms of handling sporadically missing data (average missing rate was 2.8 % with a standard deviation of 2.5%%), complete case analysis was executed.
Then sensitivity analysis was executed.
The physicochemical parameter analysis was executed based on standard method.
Once selected, the analysis is executed on an HPC system.
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