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Preprocessing and data analysis were performed using SPM8 (www.fil.ion.ucl.ac.uk/spm).ac.uk/spm
The experiments and data analysis were performed by Beijing Boao Crystal Code Biotechnology Co. Ltd.
Several challenges during database development and data analysis were identified.
Statistics: Routinely, data collection and data analysis were performed by different persons, to blind the potential bias.
Stimulus presentation, data collection and data analysis were performed using custom software written in Matlab (MathWorks R2010b, Massachusetts, USA).
The statistics used in this data analysis were performed with the T-TEST function in Microsoft Excel.
Sequencing and data analysis were conducted as previously described with ImmunoSEQ assay by Adaptive Biotechnologies Corp43,44.
Preprocessing and data analysis were performed using Statistical Parametric Mapping software implemented in Matlab (SPM8; Wellcome Department of Cognitive Neurology, London, UK).
Author Contributions Experimental work and data analysis were performed by M.J.G. and N.J.T. C.R.T. performed all immunological work and associated data analysis.
Methylation profiles from the Infinium 450K array data analysis were highly concordant with those from the WGBS analysis (Supplementary Figure S2).
Amplification, detection, and data analysis were carried out with an ABI PRISM 7900HT Sequence Detection System and normalized to GUSB and GAPDH expression.
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