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Data analysis was executed using Ultrascan II [34] where 3 scans at 3 different speeds were simultaneously fitted to a one-component model.
Data analysis was executed in analogy to Probe based Real-Time PCR.
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Data collection and data analysis were executed iteratively.
Thus, the fitting analysis was executed with four different data sets.
A factorial map of principal component analysis was executed on the whole expressed data by the program Qlucore [ 68] on the differentially expressed (fold change >2).
In terms of handling sporadically missing data (average missing rate was 2.8 % with a standard deviation of 2.5%%), complete case analysis was executed.
Then sensitivity analysis was executed.
The physicochemical parameter analysis was executed based on standard method.
The analysis was executed using the DMU Package [26], [27].
Confirmatory factor analysis was executed to determine factorial/construct validity.
Gravimetric analysis was executed at the end of the protocol.
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