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Data analysis was accomplished using the manufacturer's software (TAM Assistant, TA Instruments, New Castle, DE, USA) and Origin 7.5 (Microcal, Northampton, MA, USA).
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All LESMD calculations were performed using NAMD version 2.6 and the CHARMM27 all atom force field, while the data analysis was accomplished using Visual Molecular Dynamics software (VMD).
Data analysis was accomplished using SAS, version 9.3 (SAS institute, Inc., Cary, NC, USA).
Data were plotted and statistical analysis was accomplished using the STATISTICA for Windows computer program (StatSoft, Inc. Tulsa, OK).
Western blot analysis was accomplished using the infrared imaging system, Odyssey (Li-Cor, Inc).
Plots were done using R, and regression analysis was accomplished using the lme4 package [ 51].
Maximum Likelihood analysis was accomplished using the RaxML BlackBox webserver [ 28].
Mutational analysis was accomplished using the Sequenom Typer Analyzer 4.0 software (Sequenom, San Diego, CA).
Score translation and ROC analysis was accomplished using the R-package "rms" [ 22].
Our initial analysis was accomplished using the program PILER (Edgar and Myers 2005).
The statistical analysis was accomplished using SPSS 13.0.
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