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Data analyses were calculated using SAS 8.0 and 9.1 (SAS, Cary, NC, USA) and EpiInfo version 3.4 (Centers for Disease Control and Prevention, Atlanta, GA, USA).
Data analyses were calculated using SAS Version 9.1.3 (SAS Institute, Cary, NC, USA) and all P-values were two-tailed with an α level of 0.05.
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Age data for all analyses were calculated based on self-reported date of birth from the study questionnaire.
All data, graphs and statistical analyses were calculated with Microsoft Excel (Microsoft Office Software, Reading, UK), GraphPad Prism (GraphPad Software Inc., La Jolla, CA, USA) and ImageJ.
Data summaries and descriptive statistical analyses were calculated using Microsoft Excel X for Mac Service Release 1 (Microsoft Corporation).
All co-localization analyses were calculated using data obtained from at least 2 separate fields from an n of 4 mice.
Statistical analyses were calculated on the smoothed data with a high-pass filter (128 s cutoff period) to remove low-frequency noise.
Statistical analyses were calculated on the smoothed data (4 × 4 × 8 mm Gaussian kernel).
Linear regression analyses were calculated using Excel.
To assure the same exposure limits between tertiles in the two sets of analyses, data analyses were based on tertiles calculated from the exposure distribution in the conception cycle.
Data analyses were manually performed to calculate the key variables including PCR efficiency (E = (10exp[-1/slope]-1)×100) and squared correlation coefficients (R) of primer sets and expression ratios of target genes were normalized according to expression levels of RP49.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com