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The following paper provides a summary of some primary design features of the 26-m tall test facility along with a description of the data acquisition suite that guides our experimental practices.
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After microarray raw data acquisition, data processing was performed using the web-accessible Micro Array Data Suites of Computed Analysis, MADSCAN resource (at [ 105]; [ 106]).
To achieve this goal, we developed a new data acquisition and analysis suite.
Data acquisition was performed with Micro Array Suite 5.0 software (Affymetrix, Santa Clara, CA, USA).
Menegas et al. had to develop a suite of new data acquisition and analysis tools to map the 3D position of the fluorescently labeled neurons and align their results across animals.
Following data acquisition, the scanned images were quantified using Microarray Suite 5.0 (MAS 5.0) software (Affymetrix, Santa Clara, CA) yielding a signal intensity for each probe on the GeneChip.
Affymetrix Microarray Suite 5.0 (Affymetrix) was used for initial data acquisition and analysis.
Data acquisition and analysis were done on a PC using pClamp 8 software suite (Axon) and a signal interface (DigiData 1200B, Axon) running at a sampling frequency of 20 kHz.
Each PhyloChip was scanned and recorded as a pixel image, and initial data acquisition and intensity determination were performed using standard Affymetrix software (GeneChip microarray analysis suite, version 5.1).
Data acquisition system.
Data acquisition board.
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