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The data acquisition duration was relatively short at 87 seconds, which may limit the assessment of the full wash-out phase and thus the influence the parameter derivation.
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In addition, a long data-acquisition duration is also required to minimize contamination from inelastic deformation or artificial sources.
8 Both the ZS and BIRD methods are typically used to construct a pure shift interferogram, which can be Fourier transformed to yield a decoupled spectrum from a series of short chunks of data acquisition of duration 1/SW1; 3a real-time windowed acquisition can sometimes be used to speed up experiments, but at some cost to spectral quality and resolution.
While in our study, the IMCT content within the SOL muscle could not be assessed due to time constraints in the data acquisition (overall duration of the scanning protocol ~90 min), these data reflect a ~50% increase in the overall amount of non-contractile tissues in the Triceps Surae complex.
The dynamic exclusion option was enabled during data acquisition with exclusion duration of 60 seconds.
The duration of the first injection phase was determined in a similar way to that for single phase CTA, and was calculated from the duration of CT data acquisition plus 8 s, the duration of the post-threshold delay for bolus triggering.
This analysis was performed on the original PET data set comprising 300-s acquisition duration per bed position.
The above data show that reduction of acquisition duration results in an increase in likelihood of halo artefacts in our setup, especially with durations lower than 180 s per bed position.
In order to further investigate the role of MLAA arm completion, we investigated differences in the resulting attenuation map when varying the duration of data acquisition.
Although there was no significant difference in the mean z-axis coverage between group 1 and 2, the mean duration of data acquisition was significantly shorter in group 2 (0.99 ± 0.09 vs. 1.77 ± 0.22; P < 0.0001), and the mean DLP was significantly lower in group 2 (125.0 ± 36.7 vs. 150.02 ± .58.6; P < 0.0001).
Animals were anesthetized using isoflurane (1.5 3% in O2) for the duration of data acquisition.
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