Data acquisition and cell sorting were performed on FACS LSRII and FACS AriaIII (BD Biosciences).
Data were collected using the data acquisition program CELL Questpro and fluorescence was measured using an FL-1H detector as previously described [ 38].
For Raman data acquisition, the cells were air dried and stored at room temperature.
Data acquisition (∼5000 cells) was carried out using a FACSCAN flow cytometer (Becton Dickinson Biosciences, Le Pont de Claix, France) equipped with a 15-mW argon ion laser tuned at 488 nm, using the Cell Quest Pro software (Becton Dickinson Biosciences).
In super-resolution microscopy, data acquisition on a cell-by-cell basis, as well as image processing, is relatively time-consuming.
Those are precise position control of a cell dish holder, design of the cell dish, data acquisition of microscopic image of a cell organelle (cell nucleus), data processing, reliable particle detection, soft and hard wares to integrate all these related data and system to control and irradiate a targeted spot with exactly determined number of particles.
It also allows PC-based data acquisition of fuel cell power, temperature, pressure and others.
Those are precise position control of a cell dish holder, design of the cell dish, data acquisition of the microscopic image of a cell organelle (cell nucleus), data processing, reliable particle detection, software and hardware to integrate all these related data, and a system to control and irradiate a targeted spot with an exactly determined number of particles.
We discuss the considerations of using our system in various plant model systems, the use of single versus multiple expression cassettes, the application of our vectors using various transformation methods and the use of internal fluorescent markers which can assist in signal localization and easy data acquisition in living cells.
Cells were analysed in a FACScalibur flow cytometer, (Becton Dickinson, San Jose, CA, USA), data acquisition was performed with Cell Quest programme (Becton Dickinson), analysed with Modfit LT programme (Verity Software House, Topsham, ME, USA).
